《Isolation of extracellular vesicles from intestinal tissue in a mouse model of intestinal ischemia/reperfusion injury》 was written by Chen, Xiao-Dong; Zhao, Jin; Yan, Zhengzheng; Zhou, Bo-Wei; Huang, Wen-Fang; Liu, Wei-Feng; Li, Cai; Liu, Ke-Xuan. HPLC of Formula: 13395-16-9 And the article was included in BioTechniques in 2020. The article conveys some information:
Extracellular vesicles (EVs) are small membranous particles that contribute to intercellular communications. Separating EVs from tissue is still a tech. challenge. Here, we present a rigorous method for extracting EVs from intestinal tissue in a mouse intestinal ischemia/reperfusion (I/R) model, and for analyzing their miRNA content. The isolated EVs show a typical cup shape with a size peak of 120-130 nm in diameter, confirmed by TEM and NTA. They also express EV markers such as CD9, CD63, CD81, Tsg101 and Alix. Real-time qPCR confirmed that these pellets contain miRNAs related to I/R injury. Our study presents a practical way to isolate EVs from intestinal tissue which is suitable for downstream applications such as miRNA anal., and provides a novel method for investigating the mechanism of intestinal I/R injury. METHOD SUMMARY : We present a practical way to isolate EVs from intestinal tissue; the method consists of enzymic digestion, differential centrifugation and d. gradient centrifugation. This method is suitable for downstream studies such as miRNA anal. After reading the article, we found that the author used Bis(acetylacetone)copper(cas: 13395-16-9HPLC of Formula: 13395-16-9)
Bis(acetylacetone)copper(cas: 13395-16-9) is used as PVC stabilizer, and curing agents for epoxy resins, acrylic adhesives and silicone rubbers. It is also used as solvents, lubricant additives, paint drier, and pesticides.HPLC of Formula: 13395-16-9
Referemce:
Copper catalysis in organic synthesis – NCBI,
Special Issue “Fundamentals and Applications of Copper-Based Catalysts”