In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called RP-HPLC method development and validation for the simultaneous estimation of imidapril HCl and amlodipine besylate in bulk and tablet, published in 2013-02-28, which mentions a compound: 89396-94-1, mainly applied to imidapril hydrochloride amlodipine besylate determination reversed phase HPLC, Recommanded Product: 89396-94-1.
A simple reverse phase liquid chromatog. method was developed and subsequently validated for simultaneous determination of imidapril HCl and amlodipine besylate in combination. The separation was carried out using a mobile phase consisting of 0.01M potassium dihydrogen phosphate buffer (adjusted with 0.1% phosphoric acid to pH 3.0), acetonitrile and methanol in the ratio of 50: 35: 15. The column used was Lichrospher C18, 5μm, 25 cm × 4.6 mm maintained at 40°C with flow rate of 1 mL / min using PDA detection at 210 and 237 nm for imidapril HCl and amlodipine besylate resp. The described method was linear over a concentration range of 10-100 μg/mL and 14-140 μg/mL for the assay of imidapril HCl and amlodipine besylate resp. The retention times of imidapril HCl and amlodipine besylate were found to be 4.3 and 6.7 min resp. Results of anal. were validated statistically and by recovery studies. The limit of detection (LOD) and limit of quantification (LOQ) for imidapril HCl and amlodipine besylate were found to be 0.13 and 0.28 μg/mL and 0.24 and 1.47 μg/mL resp. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of amlodipine besylate and imidapril HCl bulk drug and in its pharmaceutical dosage form.
Here is a brief introduction to this compound(89396-94-1)Recommanded Product: 89396-94-1, if you want to know about other compounds related to this compound(89396-94-1), you can read my other articles.
Reference:
Copper catalysis in organic synthesis – NCBI,
Special Issue “Fundamentals and Applications of Copper-Based Catalysts”